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Fluorescence Techniques

Fluorescent Dyes

Fluorescence Glossary
A list of fluorescence terms and definitions (courtesy of Omega Optical).
Fluorescence Database
This on-line database (courtesy of Bio-Rad) allows the user to search by name, by excitation maxima (+ or - a tolerance value) or by emission maxima (+ or - a tolerance value).

Other lists with information about fluorochrome characteristics include: a fluorochrome table at the Salk Institute Flow Cytometry lab and the spectral characteristics plots available from Molecular Probes about their dyes. See also the extensive fluorochrome lists available from Carl Zeiss, Inc and at the Olympus Microscopy Resource Center. A short PDF list of fluorescent dyes and their multi-photon characteristics is available from the University of Georgia's Center for Ultrastructural Research (pdf Adobe Acrobat reader is required to open this file)

Links to useful Chemistry Sites and Resources

FRET Software

  • FRETView is program developed by this group to reduce much of the complexity involved in obtaining FRET parameters for various donor/acceptor systems.  It integrates many of the equations used for doing FRET calculation in an easy to use interface.
  • PhotochemCAD is a program for use as a computer-aided research and teaching tool in photochemistry.  It also does some FRET calculations.

Chemistry and Photochemistry Resources

 

 

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